Research

Current research interests include the application of chromatography and mass spectrometry tools to the structural analysis of novel biomolecules in arachnid and reptile venoms and amphibian defensive skin secretions. The development of analytical techniques for metabolomic studies relevant to human disease is also if interest.

Publications:

Moore, Sara W.M., Bhat, Vikas K., Flatt, Peter, Gault, Victor and McClean, Stephen (2015) Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom. Toxicon , 101 . 48 – 54. [Journal article]

Conlon, J. Michael, Mechkarska, Milena, Radosavljevic, Gordana, Attoub, Samir, King, Jay D., Lukic, Miodrag L. and McClean, Stephen (2014) A family of antimicrobial and immunomodulatory peptides related to the frenatins from skin secretions of the Orinoco lime frog Sphaenorhynchus lacteus (Hylidae). Peptides , 56 . 132 – 140. [Journal article]

McClean, Stephen (2014) Screen-captured video feedback to enhance engagement with laboratory practical work. In: Higher Education Academy STEM Annual Conference 2014, University of Edinburgh. Higher Education Academy. 1 pp. [Conference contribution]

McClean, Stephen, Beggs, Louise B and Welch, Rob (2014) Antimicrobial activity of antihypertensive food-derived peptides and selected alanine analogues. Food Chemistry, 146 . pp. 443-447. [Journal article]

Guo, Chun-teng, McClean, Stephen, Shaw, Chris, Rao, Ping-fan, Ye, Ming-yu and Bjourson, AJ (2013) Purification, characterization and molecular cloning of chymotrypsin inhibitor peptides from the venom of Burmese Daboia russelli siamensis. Peptides (in press)

Guo, Chun-teng, McClean, Stephen, Shaw, Chris, Rao, Ping-fan, Ye, Ming-yu and Bjourson, AJ (2013) Trypsin and chymotrypsin inhibitor peptides from the venom of Chinese Daboia russellii siamensis. Toxicon, 63 . pp. 154-164.

Maguire, Orla, Pollock, Catherine, Martin, Philip, Owen, Andrew, Smyth, Thomas, Doherty, Declan, Campbell, Moray J., McClean, Stephen and Thompson, Paul (2012) Regulation of CYP3A4 and CYP3A5 expression and modulation of “intracrine” metabolism of androgens in prostate cells by liganded vitamin D receptor. Molecular and Cellular Endocrinology, TBC.

Martin, Christine M.A., Gault, Victor, McClean, Stephen, Flatt, Peter and Irwin, Nigel (2012) Degradation, insulin secretion, glucose-lowering and GIP additive actions of a palmitate-derivatised analogue of xenin-25. Biochemical Pharmacology, 84 (3). 312- 319.

Quinn, Gerry A., Maloy, Aaron P., McClean, Stephen, Carney, Brian and Slater, John W. (2012) Lipopeptide Biosurfactants from Paenibacillus polymyxa Inhibit Single and Mixed Species Biofilms. Biofouling, 28 (10). pp. 1151-1166.

McClean, Stephen (2011) Investigation of Animal Venoms and Toxins. In: Methods in Animal Proteomics. Wiley-Blackwell, pp. 211-241. ISBN 978-0813817910

Domann PJ, Akashi S, Barbas C, Huang L, Lau W, Legido-Quigley C, McClean S, Neusüss C, Perrett D, Quaglia M, Rapp E, Smallshaw L, Smith NW, Smyth WF, Taylor CF; (2010) Guidelines for reporting the use of capillary electrophoresis in proteomics. Minimum Information About a Proteomics Experiment (MIAPE).
Nature Biotechnology Jul;28(7):654-5.

Smyth, Thomas, Perfumo, A, McClean, Stephen, Marchant, Roger and Banat, Ibrahim (2010) Isolation and Analysis of Lipopeptides and High Molecular Weight Biosurfactants. In: Handbook of Hydrocarbon and Lipid Microbiology. Springer-Verlag, pp. 3688-3704. ISBN 978-3-540-77584-3

Moore, Sara, Smyth, Franklin, Gault, Victor, O’Kane, Edmund and Mcclean, Stephen (2009) Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae). RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 23 (12). pp. 1747-1755.

Stephen McClean (2008) Excel Yourself with Personalised Email Messages, Bioscience Education12-c1 available at http://www.bioscience.heacademy.ac.uk/journal/vol12/beej-12-c1.aspx

Stephen McClean (2008) Making Small Talk — Audio MP3 Files Made More Portable, Bioscience Education,12-L1 available at http://www.bioscience.heacademy.ac.uk/journal/vol12/beej-12-l1.aspx

Doherty, Bernadette, O’Donnell, Fionnuala, Smyth, Franklin, Leslie, Julian, Ramachandran, Venkataraman N., Boyd, Neil S., Hack, Catherine, O’Kane, Edmund and Mcclean, Stephen (2007) A study of the analytical behaviour of selected psycho-active drugs using liquid chromatography, ion trap mass spectrometry, gas chromatography and polarography and the construction of an appropriate database for drug characterisation. TALANTA, 72 (2). pp. 755-761.

Doherty, Bernadette, Rodriguez, Virginia, Leslie, Julian, Mcclean, Stephen and Smyth, Franklin (2007) An electrospray ionisation tandem mass spectrometric investigation of selected psychoactive pharmaceuticals and its application in drug and metabolite profiling by liquid chromatography/electrospray ionisation tandem mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21 (13). pp. 2031-2038.

Rao, J. R., Nelson, D. W. A. and McClean, Stephen (2007) The enigma of double-stranded RNA (dsRNA) associated with mushroom virus X (MVX). Current Issues in Molecular Biology, 9 . pp. 103-121.

Thompson, Alan Hunter, Bjourson, AJ, Orr, David Francis, Shaw, Chris and McClean, Stephen (2007) Amphibian skin secretomics: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea: Discovery of a novel peptide family, the hyposins. JOURNAL OF PROTEOME RESEARCH, 6 (9). pp. 3604-3613.

Thompson, Alan Hunter, Bjourson, AJ, Orr, David Francis, Shaw, Chris and McClean, Stephen (2007) A combined mass spectrometric and cDNA sequencing approach to the isolation and characterization of novel antimicrobial peptides from the skin secretions of Phyllomedusa hypochondrialis azurea. PEPTIDES, 28 (7). pp. 1331-1343.

Zhou, J. W., Bjourson, AJ, Coulter, Danny, Chen, T. B., Shaw, C., O’Rourke, M., Hirst, D. G., Zhang, Y. N., Rao, P. F. and McClean, Stephen (2007) Bradykinin-related peptides, including a novel structural variant, (Val(1))-bradykinin, from the skin secretion of Guenther’s frog, Hylarana guentheri and their molecular precursors. Peptides, 28 (4). pp. 781-789.

Graham, C, Richter, SC, McClean, Stephen, O’Kane, E, Flatt, Peter and Shaw, C (2006) Histamine-releasing and antimicrobial peptides from the skin secretions of the Dusky Gopher frog, Rana sevosa. PEPTIDES, 27 (6). pp. 1313-1319.

Graham, C., Richter, S. C., McClean, Stephen, O’Kane, Ej, Flatt, Peter and Shaw, C. (2006) Histamine-releasing and antimicrobial peptides from the skin secretions of the Dusky Gopher frog, Rana sevosa. Peptides, 27 (6). pp. 1313-1319.

Rustam, Tarick, Mcclean, Stephen, Newcombe, Jane, McFadden, Johnjoe and Eales-Reynolds, Lesley-Jane (2006) Reduced toxicity of lipo-oligosaccharide from a phoP mutant of Neisseria meningitidis: an in vitro demonstration. Journal of Endotoxin Research, 12 (1). pp. 39-46.

Smyth, Franklin, Leslie, Julian, Mcclean, Stephen, Hannigan, Bernie, Mckenna, Hugh, Doherty, B, Joyce, C and O’Kane, E (2006) The characterisation of selected antidepressant drugs using electrospray ionisation with ion trap mass spectrometry and with quadrupole time-of-flight mass spectrometry and their determination by high-performance liquid chromatography/electrospray ionisation tandem mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20 (11). pp. 1637-1642.

Smyth, Franklin, Mcclean, Stephen, Hack, Catherine, Ramachandran, VN, Doherty, B, Joyce, C, O’Donnell, F, Smyth, Thomas, O’Kane, E and Brooks, P (2006) The characterisation of synthetic and natural-product pharmaceuticals by electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 25 (6). pp. 572-582.

Thompson, Alan Hunter, Bjourson, AJ, Shaw, Chris and Mcclean, Stephen (2006) Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: mass spectrometric structural characterisation and cloning of precursor cDNAs. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20 (24). pp. 3780-3788.

Zhou, Jianwu, Mcclean, Stephen, Thompson, Alan, Zhang, Yang, Shaw, Chris, Rao, Pingfan and Bjourson, AJ (2006) Purification and characterization of novel antimicrobial peptides from the skin secretion of Hylarana guentheri. PEPTIDES, 27 (12). pp. 3077-3084.

Graham, C, Irvine, AE, McClean, Stephen, Richter, SC, Flatt, Peter and Shaw, C (2005) Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa. PEPTIDES, 26 (5). pp. 737-743.

Graham, R. L., McClean, Stephen, O’Kane, Ej, Theakston, D. and Shaw, C. (2005) Adenosine in the venoms from viperinae snakes of the genus Bitis: identification and quantitation using LC/MS and CE/MS. Biochemical and biophysical research communications, 333 (1). pp. 88-94.

Graham, R. L. J., Graham, C., McClean, Stephen, Chen, T., O’Rourke, M., Hirst, D., Theakston, D. and Shaw, C. (2005) Identification and functional analysis of a novel bradykinin inhibitory peptide in the venoms of New World Crotalinae pit vipers. Biochemical and biophysical research communications, 338 (3). pp. 1587-1592.

Zheng, Huiru, Ojha, Piyush, McClean, Stephen, Graham, C., Black, Norman, Hughes, J. G. and Shaw, C. (2005) A computational approach for peptidomic analysis in taxonomic study of amphibian species. Ieee Transactions on Nanobioscience, 4 (3). pp. 241-247.

Chen, TB, Orr, DF, O’Rourke, M, McLynn, C, Bjourson, AJ, Mcclean, Stephen, Hirst, D, Rao, PF and Shaw, C (2004) Pachymedusa dacnicolor tryptophyllin-1: structural characterization, pharmacological activity and cloning of precursor cDNA. REGULATORY PEPTIDES, 117 (1). pp. 25-32.

Marenah, L, Flatt, Peter, Orr, DF, McClean, Stephen, Shaw, C and Abdel-Wahab, Yasser (2004) Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures. BIOLOGICAL CHEMISTRY, 385 (3-4). pp. 315-321.

Marenah, L, McClean, Stephen, Flatt, Peter, Orr, DF, Shaw, C and Abdel-Wahab, Yasser (2004) Novel insulin-releasing peptides in the skin of Phyllomedusa trinitatis frog include 28 amino acid peptide from dermaseptin BIV precursor. PANCREAS, 29 (2). pp. 110-115.

Marenah, L, Shaw, C, Orr, DF, McClean, Stephen, Flatt, Peter and Abdel-Wahab, Yasser (2004) Isolation and characterisation of an unexpected class of insulinotropic peptides in the skin of the frog Agalychnis litodryas. REGULATORY PEPTIDES, 120 (1-3). pp. 33-38.

Marenah, L., Flatt, Peter, Orr, D. F., McClean, Stephen, Shaw, C. and Abdel-Wahab, Yasser (2004) Brevinin-1 and multiple insulin-releasing peptides in the skin of the frog Rana palustris. Journal of Endocrinology, 181 (2). pp. 347-354.

Zheng, Huiru, Ojha, Piyush, McClean, Stephen, Black, Norman, Hughes, J. G. and Shaw, C. (2004) Peptidomic pattern analysis and taxonomy of amphibian species. Computational and Information Science, Proceedings, 3314 . pp. 498-503.

Chen, TB, Bjourson, AJ, McClean, Stephen, Orr, DF, O’Kane, EJ, Rao, PF and Shaw, C (2003) Cloning of maximakinin precursor cDNAs from Chinese toad, Bombina maxima, venom. PEPTIDES, 24 (6). pp. 853-861.

Gallizia, I, McClean, Stephen and Banat, Ibrahim (2003) Bacterial biodegradation of phenol and 2,4-dichlorophenol. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 78 (9). pp. 959-963.

Lundy, F. T., Orr, D. F., McClean, Stephen, Shaw, S., Lamey, P. J. and Linden, G. J. (2003) Enzyme catabolism of calcitonin gene-related peptide in periodontitis. Journal of dental research, 82 . p. 570.

Zheng, Huiru, Ojha, Piyush, McClean, Stephen, Black, Norman, Hughes, J. G. and Shaw, C. (2003) Heuristic charge assignment for deconvolution of electrospray ionization mass spectra. Rapid communications in mass spectrometry : RCM, 17 (5). pp. 429-436.

Chen, TB, Orr, DF, Bjourson, AJ, Mcclean, Stephen, O’Rourke, M, Hirst, DG, Rao, PF and Shaw, C (2002) Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis). PEPTIDES, 23 (9). pp. 1547-1555.

Chen, TB, Orr, DF, Bjourson, AJ, Mcclean, Stephen, O’Rourke, M, Hirst, DG, Rao, PF and Shaw, C (2002) Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 (18). pp. 4693-4700.

McClean, Stephen, Robinson, R. C., Shaw, C. and Smyth, Franklin (2002) Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry. Rapid communications in mass spectrometry : RCM, 16 (5). pp. 346-354.

Rahman, KSM, Rahman, TJ, McClean, Stephen, Marchant, R and Banat, Ibrahim (2002) Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials. BIOTECHNOLOGY PROGRESS, 18 (6). pp. 1277-1281.

Conneely, A, Mcclean, Stephen, Smyth, Franklin and Mcmullan, Geoffrey (2001) Study of the mass spectrometric behaviour of phthalocyanine and azo dyes using electrospray ionisation and matrix-assisted laser desorption/ionisation. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 15 (22). pp. 2076-2084.

Mcclean, Stephen, O’Kane, EJ and Smyth, Franklin (2000) Electrospray ionisation-mass spectrometric characterisation of selected anti-psychotic drugs and their detection and determination in human hair samples by liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 740 (2). pp. 141-157.

Mcclean, Stephen, O’Kane, EJ and Smyth, Franklin (2000) The identification and determination of selected 1,4-benzodiazepines by an optimised capillary electrophoresis – electrospray mass spectrometric method. ELECTROPHORESIS, 21 (7). pp. 1381-1389.

Smyth, Franklin, McClean, Stephen and Ramachandran, V. N. (2000) A study of the electrospray ionisation of pharmacologically significant 1,4-benzodiazepines and their subsequent fragmentation using an ion-trap mass spectrometer. Rapid communications in mass spectrometry : RCM, 14 (21). pp. 2061-2069.

McClean, Stephen, O’Kane, Ej, Hillis, J. and Smyth, Franklin (1999) Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry. Journal of chromatography.A, 838 (1-2). pp. 273-291.

Smyth, Franklin, McClean, Stephen, O’Kane, Ej, Banat, Ibrahim and Mcmullan, Geoffrey (1999) Application of electrospray mass spectrometry in the detection and determination of Remazol textile dyes. JOURNAL OF CHROMATOGRAPHY A, 854 (1-2). pp. 259-274.

McClean, Stephen, O’Kane, Ej, Coulter, Danny, McLean, S. and Smyth, Franklin (1998) Capillary electrophoretic determination of trace metals in hair samples and its comparison with high performance liquid chromatography and atomic absorption spectrometry techniques. Electrophoresis, 19 (1). pp. 11-18.

Smyth, Franklin and McClean, Stephen (1998) A critical evaluation of the application of capillary electrophoresis to the detection and determination of 1,4-benzodiazepine tranquilizers in formulations and body materials. Electrophoresis, 19 (16-17). pp. 2870-2882.

Harland, G. B., McGrath, G., McClean, Stephen and Smyth, Franklin (1997) Use of large volume sample stacking for improving limits of detection in the capillary electrophoretic determination of selected cations. Analytical Communications, 34 (1). pp. 9-11.

Harland, GB, McGrath, G, McClean, Stephen and Smyth, Franklin (1997) Use of large volume sample stacking for improving limits of detection in the capillary electrophoretic determination of selected cations. ANALYTICAL COMMUNICATIONS, 34 (1). pp. 9-11.

Smyth, Franklin, Harland, G. B., McClean, Stephen, McGrath, G. and Oxspring, D. (1997) Effect of on-capillary large volume sample stacking on limits of detection in the capillary zone electrophoretic determination of selected drugs, dyes and metal chelates. Journal of Chromatography a, 772 (1-2). pp. 161-169.

McGrath, G., McClean, Stephen, O’Kane, Ej, Smyth, Franklin and Tagliaro, F. (1996) Study of the capillary zone electrophoretic behaviour of selected drugs, and its comparison with other analytical techniques for their formulation assay. Journal of Chromatography a, 735 (1-2). pp. 237-247.

Oxspring, D. A., McClean, Stephen, O’Kane, Ej and Smyth, Franklin (1995) Study of the chelation of boron with Azomethine H by differential pulse polarography, liquid chromatography and capillary electrophoresis and its analytical applications. Analytica Chimica Acta, 317 (1-3). pp. 295-301.

Mcclean, Stephen, MAXWELL, T, RAMACHANDRAN, VN, OKANE, E and Smyth, Franklin (1994) A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS. ELECTROANALYSIS, 6 (11-12). pp. 996-1003.

McClean, Stephen, Maxwell, T., Ramachandran, V. N., O’Kane, Ej and Smyth, Franklin (1994) A Study of the Differential-Pulse Polarographic and Stripping Voltametric Behavior of the H+. Electroanalysis, 6 (11-12). pp. 996-1003.

McClean, Stephen, O’Kane, Ej, Ramachandran, V. N. and Smyth, Franklin (1994) Differential-Pulse Polarographic Study of the Degradation of H+. Analytica Chimica Acta, 292 (1-2). pp. 81-89.

O’Kane, Ej, Ramachandran, V. N., McClean, Stephen and Smyth, Franklin (1993) Ultraviolet Spectrophotometric, Solvent-Extraction, High-Performance Liquid-Chromatographic and Polarographic Study of the H+. Analyst, 118 (5). pp. 511-516.

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